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His-Tag Monoclonal Antibody
$200.00
The 6×His tag is a synthetic peptide consisting of six consecutive histidine residues (HHHHHH), typically engineered at either the N- or C-terminus of recombinant proteins. This tag enables efficient purification of tagged proteins through immobilized metal affinity chromatography (IMAC), leveraging the strong and selective binding of histidine clusters to metal ions such as Ni²⁺ or Co²⁺.
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Product Specifications
| Shipping | Dry Ice |
|---|---|
| Storage | -20°C |
| Storage Information | Avoid freeze/thaw cycles |
| Species | Mouse |
| Purity | The antibody was purified by immunogen affinity chromatography. |
| Form | Liquid |
| Isotype | IgG |
| Description | Mouse monoclonal antibody to His-tag |
| Concentration | 1 mg/mL |
| Clonality | Monoclonal |
| Specificity | Recognizes C-terminal, internal, and N-terminal His-tag fusion proteins. |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence of His-tag. The exact sequence is proprietary. |
| Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Applications
- WB: 1:1000 – 1:3000
- IF/IC: 1:100 – 1:200
- IP: 1:50 – 1:200
