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Antibodies

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Epitope Tag Antibodies & HRP Conjugates

ORF Biologics supplies research antibodies for detecting tagged recombinant proteins and the secondaries that go with them. The catalog covers mouse monoclonal and rabbit polyclonal antibodies to His, FLAG, HA, Myc, GST, GFP, mCherry, MBP, Strep, T7 and VSV-G tags, plus HRP-conjugated anti-human, anti-mouse and anti-rabbit IgG. All are validated as Western blot antibodies, and many for immunofluorescence, immunoprecipitation and ELISA. Browse by application below, or go straight to primary antibodies or secondary antibodies.

Western Blot Antibodies and More, by Application

Every ORF antibody lists its validated applications and recommended dilutions on the product page. Start with what you’re running.

Western Blot

Detect tagged proteins in lysates and purified fractions. All 16 antibodies are validated for WB.

His-Tag

FLAG Tag

HA-Tag

Myc-Tag

GFP-Tag

mCherry

Strep-Tag

See all secondaries

Immunofluorescence & Immunocytochemistry

Localize tagged proteins in fixed cells.

His-Tag

HA-Tag

Myc-Tag

GST-Tag

GFP-Tag

VSV-G-Tag

Immunoprecipitation

Pull down tagged proteins and their binding partners.

His-Tag

HA-Tag

Myc-Tag

GST-Tag

GFP-Tag

VSV-G-Tag

ELISA & IHC

HRP-conjugated anti-IgG secondaries for enzyme-based detection.

Goat Anti-Human IgG HRP

Goat Anti-Mouse IgG HRP

Goat Anti-Rabbit IgG HRP

Rabbit Anti-Human IgG HRP

Rabbit Anti-Mouse IgG HRP

Antibodies Built for Clean, Reproducible Blots

ORF Biologics antibodies are affinity-purified and tested for specificity, lot-to-lot consistency and low background before they ship.

Affinity Purified — Every antibody is purified by antigen or immunogen affinity chromatography, so what’s in the vial is the antibody that binds your tag.

Specificity Tested — Tag antibodies recognize native and denatured fusion proteins. Secondaries show no detected reactivity with non-immunoglobulin serum proteins.

Validated Applications — Tested in Western blot and, where listed, IF, IP, ELISA, and IHC, with recommended dilutions on every product page.

Lot-to-Lot Consistency — Each lot is held to the same specification for consistent results from one experiment to the next.

ORF Biologics is a U.S.-based antibody supplier in Tiffin, Iowa. Antibodies ship on dry ice at 1 mg/mL in glycerol-stabilized buffer and store at -20ºC.

Compare Epitope Tag Antibodies and HRP Secondaries

Tag antibodies are supplied in 50 µg and secondaries in 100 µg, all at 1 mg/mL. Click any product for full specifications, buffer, and dilution ranges.

AntibodyHost / clonalityValidated applicationsSize
His-Tag Monoclonal AntibodyMouse monoclonalWB, IF/IC, IP50 µg
FLAG-Tag Monoclonal AntibodyMouse monoclonalWB50 µg
HA-Tag Monoclonal AntibodyMouse monoclonalWB, IF/IC, IP50 µg
Myc-Tag Monoclonal AntibodyMouse monoclonalWB, IF/IC, IP50 µg
GST-Tag Monoclonal AntibodyMouse monoclonalWB, IP50 µg
GFP-Tag Monoclonal AntibodyMouse monoclonalWB, IF/IC, IP50 µg
T7-Tag Monoclonal AntibodyMouse monoclonalWB50 µg
VSV-G-Tag Monoclonal AntibodyMouse monoclonalWB, IF/IC, IP50 µg
mCherry Polyclonal AntibodyRabbit polyclonalWB50 µg
MBP Polyclonal AntibodyRabbit polyclonalWB50 µg
Strep-Tag Polyclonal AntibodyRabbit polyclonalWB50 µg
Goat Anti-Human IgG (H&L) HRPGoat polyclonal, HRPELISA, WB, IHC, ICC100 µg
Goat Anti-Mouse IgG (H&L) HRPGoat polyclonal, HRPELISA, WB, IHC, ICC100 µg
Goat Anti-Rabbit IgG (H&L) HRPGoat polyclonal, HRPELISA, WB, IHC, ICC100 µg
Rabbit Anti-Human IgG (H&L) HRPRabbit polyclonal, HRPELISA, WB, IHC, ICC100 µg
Rabbit Anti-Mouse IgG (H&L) HRPRabbit polyclonal, HRPELISA, WB, IHC, ICC100 µg

Antibody FAQs

Which antibody should I use to detect a His-tagged protein?

The His-Tag Monoclonal Antibody recognizes N-terminal, C-terminal and internal 6×His tags on both native and denatured proteins, and is validated for Western blot, IF/IC and IP. Pair it with Goat Anti-Mouse IgG HRP for chemiluminescent detection.

Are ORF antibodies validated for Western blot?

Yes. Every antibody in the catalog is validated as a Western blot antibody, with a recommended dilution range on its product page. Tag monoclonals typically run at 1:1,000 to 1:5,000 and HRP secondaries at 1:5,000 to 1:20,000.

What’s the difference between a tag antibody and a secondary antibody?

A tag antibody binds the epitope tag fused to your protein of interest. A secondary antibody binds the tag antibody itself and carries the HRP enzyme that generates the signal. You’ll usually need one of each: a mouse monoclonal tag antibody with an anti-mouse IgG HRP secondary, or a rabbit polyclonal with an anti-rabbit IgG HRP secondary.

How should I store and handle antibodies?

Store at −20°C and avoid repeated freeze/thaw cycles. Antibodies are supplied in liquid form at 1 mg/mL in a glycerol-containing buffer and ship on dry ice. Full buffer composition is on each product page.

Need a larger pack size or a different format?

ORF Biologics offers bulk quantities and custom formulations for labs that run the same blot every week.

Explore Custom Biologics